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The author described the experience at Kennedy Institute of Ethics during her visit and learning as a senior visiting scholar .The author also introduced Kennedy Institute of Ethics to China .In that institute , they not only have the largest library of bioethics in the world but also have a group of research experts and professors who love bioethics and treat their work diligently , show their enthusiasm and warm care to new learning scholars , etc.
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Object To explore the antitumor mechanism of Stellera chamaejasme Linn.(SC).Methods SC containing-serum(SCCS)was derived from mice pretreated with different doses of SC.Cultured human leukemia HL-60 and human gastric adenocarcinoma SGC-7901 cells were used.Inhibition of proliferation was measured using MTT assay.Morphological assessment of apoptosis was performed with fluorescence microscope.DNA fragmentation was assessed by agarose gel electrophoresis and flow cytometry.Expression of bcl-2 protein was measured with immunohistochemistry.Results Exposure of exponentially growing HL-60 cells to mice serum containing 10% SC(pretreated with SC3,6, and 12 g/kg)for 48h resulted in growth inhibition in a dose-dependent manner.Typical morphological changes of apoptosis and DNA fragmentation in HL-60 cells were induced."Apobodies'in the apoptotic cells were observed,'ladder"pattern of agarose gel electrophoresis of DNA from 11.7% to 57.4%.Treatment with SC containing serum decreased the percentage of SGC-7901 cell of bcl-2 protein positive expression from 78.3% to 32.9%.Conclusion SC could induce apoptosis of HL-60 cells and decrease the expression of bcl-2 protein of gastric adenocarcinoma SGC-7901 cells.
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In January 2004,the long expected written regulation of 'Guidelines for research on human embryonic stem cells' was jointly released by the Ministry of Science and Technology and the Ministry of Health,Beijing,China.In 'Ethical Guidelines for Research on Human Embryonic Stem Cells',The Article 5 say: The human embryonic stem cell used for research can derived from ① spared gamete or blastula remaining after In Vitro Fertilization(IVF);②fetal cells after natural or voluntarily selective abortion;③blastula or monosexual split blastula by somatic cell nucleus transfer technique;④germ cells voluntarily donated;The Article 6 say: any blastula obtained by IVF,somatic cell nucleus transfer technique,mono-sexual reproduction technique or genetic modification cannot be cultured in ex vivo for longer than 14 days,since fertilization or nucleus transfer.Such supporting to the embryo researched gotten by the somatic cell nucleus transfer technique,and supporting the human embryonic stem cell research under the condition of the embryo researched within 14 days,is meeting the objections from native country and some foreign countries.In my paper,I argued that an embryo within 14 days is not a person,an embryo is only a human biological life,a human embryo has a certain value,it deserves due to respect,but if there are enough reasons,it can be used.Destructive embryo research should only be approved in exceptional circumstances.To the blastulas or mono-sexual split blastulas by somatic cell nucleus transfer technique,this is an Ethical issue of creating embryos for research.The majority of bioethicists said it is wrong to create and destroy a person for research.I argued that the Moral Status of Researched Human Embryo is not a person,so, Kant' theory is no used here.I further claimed that a 14 days old embryo could be used for research.I quoted a major view of the moral status of the embryo/fetus from the medical science.A 14 days old embryo is a cluster of cells without bones,organs of other traits;they have a consciousness around 20 weeks,so a 14 days old embryo cannot be hurt.
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With the Human Genome Program coming to a successful end,the post genomic era has arrived in which the research of post genomic program is developing quickly.In this paper,the developing trend of the functional genomics research in post genomic era are discussed,with the contents involved: functional genomics,functional proteomics,pharmacogenomics,gene testing,bioinformatics,comparative genomics,which they are main research fields of post genomic era.In the last part,related ethical,legal and social implications are discussed.
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Object To explore, on the whole, the anticancer activity and mechanism of the aqueous extract of Ruixiang Langdu (Stellera chamaejasme L.) (SCLA). Methods Mice were given different i.g. doses of SCLA and their serum collected at different intervals after drug administration. The effects of the serum on the proliferation of mouse L 1210 leukemic cells were observed with MTT assay, clone formation and incorporation of [ 3H] TdR into DNA of the cells. Results Serum SCLA collected 1, 2, 4, 8 h after administration of 3, 6, and 12 g/kg SCLA showed significant decrease of MTT formazans and clone formation. Serum SCLA collected 2 h after drug administration showed more strong inhibition of cancer cell proliferation. It also inhibited the incorporation of [ 3H] TdR into DNA of L 1210 cells. Conclusion SCLA showed a remarkable inhibitory effect on proliferation and DNA synthesis of cancer cells cultured in vitro, which may be the main anticancer mechanisms of SCLA.
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Object To explore the antitumor mechanism of Stellera chamaejasme Linn. (SC). Methods SC containing-serum (SCCS) was derived from mice pretreated with different doses of SC. Cultured human leukemia HL-60 and human gastric adenocarcinoma SGC-7901 cells were used. Inhibition of proliferation was measured using MTT assay. Morphological assessment of apoptosis was performed with fluorescence microscope. DNA fragmentation was assessed by agarose gel electrophoresis and flow cytometry. Expression of bcl-2 protein was measured with immunohistochemistry. Results Exposure of exponentially growing HL-60 cells to mice serum containing 10% SC (pretreated with SC 3, 6, and 12 g/kg) for 48 h resulted in growth inhibition in a dose-dependent manner. Typical morphological changes of apoptosis and DNA fragmentation in HL-60 cells were induced. "Apobodies" in the apoptotic cells were observed, "ladder" pattern of agarose gel electrophoresis of DNA from these cells was revealed, and the percentage of apoptotic cells with fractional DNA content increased from 11.7% to 57.4%. Treatment with SC containing serum decreased the percentage of SGC-7901 cells of bcl-2 protein positive expression from 78.3% to 32.9%. Conclusion SC could induce apoptosis of HL-60 cells and decrease the expression of bcl-2 protein of gastric adenocarcinoma SGC-7901 cells.